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mhc class ii fitc (Bio-Rad)

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Bio-Rad mhc class ii fitc
Mhc Class Ii Fitc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+mhc+ii/Mouse+anti+Horse+MHC+Class+II+Monomorphic/pm41967197-79-34-37
Average 93 stars, based on 73 article reviews

mhc class ii fitc - by Bioz Stars, 2026-10

93/100 stars

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Immunopeptidomics:

Article Title: Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs
Article Snippet: After the final wash, cells were suspended in cell-culture medium [Iscove's modified Dulbecco Medium (IMDM), Gibco, Rockville, MD, supplemented with 2 m m sodium pyruvate (Sigma), 0·04 m m β-mercaptoethanol (Sigma), penicillin and streptomycin, 5% (v/v) heat-inactivated normal canine serum and 5% (v/v) heat-inactivated fetal bovine serum (Sigma)]. .. Antibodies and other reagents The following antibodies were used: mouse anti-canine CD3 (CA17.2A1, Instruchemie, Bilthoven, the Netherlands), mouse anti-canine CD3-fluourescein isothiocyanate (FITC; CA17.2A1, Serotec, Raleigh, NC), rat anti-canine CD4 (YKIX302.9, Serotec), rat anti-canine CD4-FITC (YKIX302.9, Instruchemie), rat anti-canine CD8R-phycoerythrin (PE; YCATE55, Serotec), mouse anti-MHC II (CVS20, Serotec), rat anti-canine MHC II (YKIX.344, Instruchemie), mouse anti-CD14R-PE-Cy5 (TUK4, Instruchemie), mouse anti-CD21-PE (B-Ly4, Pharmingen, San Diego, CA), rat anti-canine CD45 (YKIX716.1, Serotec), mouse anti-canine CD45RA (CA.1D3, P. Moore), rat anti-mouse IgG1-FITC (A85-1, Pharmingen), rat anti-mouse IgG1-biotin (LO-MG1-1, Biosource, Camarillo, CA), rat anti-mouse IgG1-PE (X56, Becton Dickinson, San Jose, CA), mouse anti-rat IgG2a-biotin (RG7/1.30, Pharmingen), mouse anti-rat IgG2b-biotin (RG7/11.1, Pharmingen), and corresponding isotype controls (Becton Dickinson and Pharmingen). .. SA-RPE-Cy5 was purchased from DAKO (Carpenteria, CA), and SA-FITC and SA-RPE were purchased from Becton Dickinson.

Article Title: Neurodegeneration and Glial Response after Acute Striatal Stroke: Histological Basis for Neuroprotective Studies
Article Snippet: .. Activated microglia/macrophages were labeled using the antibody anti-rat CD68 (clone ED1, 1 : 500, Serotec, UK), which binds to an epitope on the lysosomal membrane of activated macrophages/microglia [ – ], rabbit anti-Iba1 (1 : 1000, WAKO), an antibody that recognizes a calcium binding protein present in the cytoplasm of microglia [ – ], and mouse anti-MHC-II (1 : 100, Serotec), an antibody that recognizes the major histocompatibility complex class II molecule [ ]. .. Based on previous reports showing that pathological oligodendrocytes become Tau-1 positive after brain trauma and ischemia [ ], we used the mouse anti-Tau-1 antibody (1 : 500, Chemicon, USA) to label dephosphorylated epitopes on damaged oligodendrocytes.

Incubation:

Article Title: Long-term accumulation of microglia with proneurogenic phenotype concomitant with persistent neurogenesis in adult subventricular zone after stroke.
Article Snippet: GLIA 20 min in 3% hydrogen peroxide and 10% methanol, and before staining with BrdU, sections were incubated in 1 M HCl at 65 C for 10 min and at room temperature for 20 min. After preincubation with appropriate normal sera, sections were incubated with rabbit anti-Iba1 (1:1000; Wako Chemicals, Osaka, Japan) overnight. .. The sections were then incubated with one of the following antibodies: mouse anti-ED1 (1:200; Serotec, Oxford, UK), mouse anti-Mac-2 (1:100, Abcam, Cambridge, UK), mouse anti-CD45 (1:50; BD Biosciences, San Jos e, CA), rat anti-BrdU (1:100; Oxford Biotechnology, Oxford, UK), mouse anti-MHC-II (1:100; Serotec), mouse antiCD86 (1:50; Serotec), mouse anti-CD43 (1:200; Serotec), mouse anti-CD8 (1:100; Serotec), mouse anti-CD4 (OX35; 1:100, Serotec), mouse anti-TCR (1:100; Serotec) and mouse anti-CD43 (1:200; Serotec), mouse anti-insulin-like growth factor-1 (IGF-1, 1:50; R&D Systems, Minneapolis, MN), goat anti-IGF-1 (1:50; Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-NeuN (1:100; Temecula, CA) and goat anti-doublecortin (Dcx, 1:400; Santa Cruz Biotechnology, Santa Cruz, CA). .. The stainings were visualized by incubation for 2 h with Cy3-conjugated donkey anti-rabbit antibody (1:200; Jackson Immunoresearch, West Grove, PA) and biotinylated secondary antibodies (1:200; Vector, Burlingame, CA) followed by Alexa 488-conjugated streptavidin (1:200; Molecular Probes, Eugene, OR), for 2 h for double stainings, or with avidin-biotin-peroxidase complex for 1 h followed by treatment with DAB (0.5 mg/mL).

Article Title: Repeated Electroconvulsive Seizures Increase the Number of Vessel-Associated Macrophages in Rat Hippocampus
Article Snippet: Objectives:We have previously reported that electroconvulsive seizure (ECS)Van animal model of the antidepressant treatment electroconvulsive therapyVcauses glial cell activation in hippocampus and other limbic areas.. In the current study, we have investigated whether the cellular response to ECS includes recruitment and infiltration of nonresident macrophages into the hippocampal brain parenchyma.. Methods: Adult rats received 1 ECS daily for 10 consecutive days and were then killed at different time points after the last ECS treatment.

Article Title: Helicobacter pylori infection negatively influences pregnancy outcome in a mouse model.
Article Snippet: Background.. Helicobacter pylori infects the human stomach, causing gastritis, peptic ulcer, and gastric cancer.. H. pylori infection has also been related to extra-gastric disorders.

Immunohistochemistry:

Article Title: Subtle thinning of retinal layers without overt vascular and inflammatory alterations in a rat model of prediabetes
Article Snippet: Rabbit anti-Iba-1 , Immunohistochemistry , 1:1000 , _ , Wako. .. Mouse anti-MHC-II , Immunohistochemistry , 1:200 , _ , AbD Serotec. ..

Labeling:

Membrane:

Binding Assay:

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$Representative image of H&E staining MPO immunohistochemistry on FFPE sections of the same tumors described in . CT26 tumors were collected 24 hours after a three intratumoral injections of PBS (50 µL) ( A ) or IL-2-OMVs Δ60 + TNFα-OMVs Δ60 (10 µg in 50 µL PBS) ( B ).( C - D - E ) Flow cytometry analysis of tumors – BALB/c mice were challenged with CT26 and when tumors reached a size of approximately 100 mm 3 mice were treated with one or three doses (two days apart) of the following formulations: PBS (control), 1 μg of OMVs Δ60 , 1 μg of CCL3-OMVs Δ60 , 1 μg of Flt3L-OMVs Δ60 . The day after the treatments, two tumors from each group receiving one dose (Post I) and three tumors from each group receiving three doses (Post III) were surgically removed. Tumor cells (1 x 10 6 ) were incubated with the appropriate fluorescent labelled antibodies and subsequently an alyzed by flow cytometry. Frequencies of regulatory T cells ( C ) γδ T cells ( D ) and LY6C/G + CD11b + myeloid cells ( E ) are calculated within the live, non-aggregated total cell populations. The dotted areas within the bars of panel D indicate the percentage of MHC II-positive γδ T cells. In panel E , each bar is subdivided into three portions of different color intensity. The dark tone represents the fraction of LY6C/G HIGH CD11b MEDIUM cells, the intermediate tone represents the fraction of LY6C/G MEDIUM CD11b HIGH cells and the light tone represents the fraction of myeloid cells excluded by the selected gating parameters. Finally, the dotted areas within each bar represent the fraction of each cell population which is MHC II-positive. The statistical analysis (unpaired, two-tailed Student’s t -test) shown in the right-hand graph refers to the populations of LY6C/G MEDIUM CD11b HIGH cells present in each group (indicated by the lines next to the bars).$

Journal: bioRxiv

Article Title: Cytokine-bearing Bacterial Outer Membrane Vesicles with Empowered Efficacy in Intratumoral Immunotherapy

doi: 10.64898/2026.04.02.716109

Figure Lengend Snippet: Representative image of H&E staining MPO immunohistochemistry on FFPE sections of the same tumors described in . CT26 tumors were collected 24 hours after a three intratumoral injections of PBS (50 µL) ( A ) or IL-2-OMVs Δ60 + TNFα-OMVs Δ60 (10 µg in 50 µL PBS) ( B ).( C - D - E ) Flow cytometry analysis of tumors – BALB/c mice were challenged with CT26 and when tumors reached a size of approximately 100 mm 3 mice were treated with one or three doses (two days apart) of the following formulations: PBS (control), 1 μg of OMVs Δ60 , 1 μg of CCL3-OMVs Δ60 , 1 μg of Flt3L-OMVs Δ60 . The day after the treatments, two tumors from each group receiving one dose (Post I) and three tumors from each group receiving three doses (Post III) were surgically removed. Tumor cells (1 x 10 6 ) were incubated with the appropriate fluorescent labelled antibodies and subsequently an alyzed by flow cytometry. Frequencies of regulatory T cells ( C ) γδ T cells ( D ) and LY6C/G + CD11b + myeloid cells ( E ) are calculated within the live, non-aggregated total cell populations. The dotted areas within the bars of panel D indicate the percentage of MHC II-positive γδ T cells. In panel E , each bar is subdivided into three portions of different color intensity. The dark tone represents the fraction of LY6C/G HIGH CD11b MEDIUM cells, the intermediate tone represents the fraction of LY6C/G MEDIUM CD11b HIGH cells and the light tone represents the fraction of myeloid cells excluded by the selected gating parameters. Finally, the dotted areas within each bar represent the fraction of each cell population which is MHC II-positive. The statistical analysis (unpaired, two-tailed Student’s t -test) shown in the right-hand graph refers to the populations of LY6C/G MEDIUM CD11b HIGH cells present in each group (indicated by the lines next to the bars).

Article Snippet: B) 25 μL of the following mixture of fluorescent-labeled antibodies were added to the samples: CD3-APC (BioLegend, San Diego, CA, USA), CD4-BV510 (BioLegend, San Diego, CA, USA), CD8a-PECF594 (BD Bioscience, San Jose, CA, USA), TCRγδ-PE (Miltenyi Biotech, Bergisch Gladbach, Germany), TCRαβ-PEVio770 (Miltenyi Biotech, Bergisch Gladbach, Germany) and MHC II (I-Ek)-VioBright (Miltenyi Biotech, Bergisch Gladbach, Germany).

Techniques: Staining, Immunohistochemistry, Flow Cytometry, Control, Incubation, Two Tailed Test